Six of the top 10 most mentioned tweets were about matches at the tournament, statistics from the company show.
Germany's 7-1 semi-final defeat of hosts Brazil was the most tweeted event, followed by Wayne Rooney's equaliser for England against Uruguay.
1D singer Harry Styles's tweet celebrating the band's fourth anniversary was the most retweeted.
The 23 July message - "4 years. Thank you to everyone who is involved in this. I feel so lucky to be part of it. Everyone who has worked with us thank you.." - has been retweeted more than 359,000 times.
In terms of news, the disappearance of Malaysia Airlines flight MH370 in March was the most talked event by UK users in 2014.
It was followed by the Scottish independence vote; the inquest of Mark Duggan, who was shot by police in Tottenham, north London in 2011, and the deaths of US comics Robin Williams and Joan Rivers.
The third most tweeted event overall in the UK in 2014 list was Daniel Sturridge's equaliser for England in the team's eventual defeat against Italy, followed by new year celebrations and Germany's defeat of Argentina in the World Cup final match.
The other World Cup tweets to make the top 10 list were about Croatia scoring against Brazil, and the Netherlands' 5-1 victory over Spain in their opening match, while matches involving Manchester United and Real Madrid, and 1D's best video Brit Award win in February also attracted attention.
The statistics for 2014 showed UK Twitter users had similar interests to the previous year when football and 1D dominated the top 10.
Globally, the Brazil World Cup in June and July broke records to become the most tweeted about subject in 2014.
Twitter has dubbed 2014 the year of the selfie, with worldwide statistics showing users had mentioned the word 92 million times, an increase of 500% on 2013.
Ellen DeGeneres' star-filled Oscar photo taken on a mobile phone was retweeted more than three million times - a Twitter record.
Lewis Wiltshire, director of media partnerships at Twitter UK also highlighted the selfie of Prince William and Prince Harry with internet entrepreneur Jamal Edwards at a Buckingham Palace event.
Between them 1D have five of the most followed UK accounts.
Harry Styles has the most followers at 22.8 million. The top 10 most-followed list also includes Adele, Emma Watson, Coldplay, BBC News' @BBCBreaking, and Ed Sheeran.
In global terms, singer Katy Perry is the most-followed person on Twitter (61 million), ahead of Justin Bieber (57 million) and Barack Obama (51 million).
(BBC)